Detection of potato mop-top virus capsid readthrough protein in virus particles.
نویسندگان
چکیده
Potato mop-top furovirus (PMTV) RNA 3 encodes the 20 kDa coat protein and a larger readthrough protein of 67 kDa. The readthrough protein is expressed by suppression of the amber stop codon which terminates the coat protein gene. A 21 kDa C-terminal fragment of the readthrough protein was doned, fused to glutathione S-transferase and expressed in E. coli. An antiserum prepared against purified fusion protein was used in ELISA to detect the readthrough protein in extracts of PMTV-infected leaves. Immunogold labelling studies showed that the readthrough protein was located near one extremity of some of the virus particles.
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ورودعنوان ژورنال:
- The Journal of general virology
دوره 78 ( Pt 7) شماره
صفحات -
تاریخ انتشار 1997