Detection of potato mop-top virus capsid readthrough protein in virus particles.

نویسندگان

  • G H Cowan
  • L Torrance
  • B Reavy
چکیده

Potato mop-top furovirus (PMTV) RNA 3 encodes the 20 kDa coat protein and a larger readthrough protein of 67 kDa. The readthrough protein is expressed by suppression of the amber stop codon which terminates the coat protein gene. A 21 kDa C-terminal fragment of the readthrough protein was doned, fused to glutathione S-transferase and expressed in E. coli. An antiserum prepared against purified fusion protein was used in ELISA to detect the readthrough protein in extracts of PMTV-infected leaves. Immunogold labelling studies showed that the readthrough protein was located near one extremity of some of the virus particles.

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عنوان ژورنال:
  • The Journal of general virology

دوره 78 ( Pt 7)  شماره 

صفحات  -

تاریخ انتشار 1997